Cytomegalovirus

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Cytomegalovirus
Typical "owl eye" intranuclear inclusion indicating CMV infection of a lung pneumocyte
Typical "owl eye" intranuclear
pneumocyte[1]
Virus classification Edit this classification
(unranked): Virus
Realm: Duplodnaviria
Kingdom:
Heunggongvirae
Phylum: Peploviricota
Class: Herviviricetes
Order: Herpesvirales
Family:
Orthoherpesviridae
Subfamily: Betaherpesvirinae
Genus: Cytomegalovirus
Species

See text

Synonyms[2]
  • Human cytomegalovirus group

Cytomegalovirus (CMV) (from cyto- 'cell' via

congenital CMV in infants can lead to deafness and ambulatory problems.[6]

In the medical literature, most mentions of CMV without further specification refer implicitly to human CMV. Human CMV is the most studied of all cytomegaloviruses.[7]

MX2/MXB was identified as a restriction factor for herpesviruses, which acts at a very early stage of the replication cycle and MX2/MXB restriction of herpesvirus requires GTPase activity.[8]

Taxonomy

Within the

varicella-zoster virus, and the Gammaherpesvirinae subfamily, which includes Epstein–Barr virus and Kaposi's sarcoma-associated herpesvirus.[7]

Several species of Cytomegalovirus have been identified and classified for different

chimpanzees and orangutans, and Simian cytomegalovirus (SCCMV) and Rhesus cytomegalovirus (RhCMV) that infect macaques; CCMV is known as both Panine beta herpesvirus 2 (PaHV-2) and Pongine betaherpesvirus 4 (PoHV-4).[10] SCCMV is called cercopithecine betaherpesvirus 5 (CeHV-5)[11] and RhCMV, Cercopithecine betaherpesvirus 8 (CeHV-8).[12] A further two viruses found in the night monkey are tentatively placed in the genus Cytomegalovirus, and are called Herpesvirus aotus 1 and Herpesvirus aotus 3. Rodents also have viruses previously called cytomegaloviruses that are now reclassified under the genus Muromegalovirus; this genus contains Mouse cytomegalovirus (MCMV) is also known as Murid betaherpesvirus 1 (MuHV-1) and the closely related Murid betaherpesvirus 2 (MuHV-2) that is found in rats.[13]

Species

The following 11 species are assigned to the genus in ICTV 2022:[5]

Structure

Schematic of a Cytomegalovirus

Viruses in Cytomegalovirus are enveloped, with icosahedral, spherical to pleomorphic, and round geometries, and T=16 symmetry. The diameter is around 150–200 nm. Genomes are linear and nonsegmented, around 200 kb in length.[4]

Genus Structure Symmetry Capsid Genomic arrangement Genomic segmentation
Cytomegalovirus Spherical pleomorphic T=16 Enveloped Linear Monopartite

Genome

Class E genome of HCMV. The unique long and unique short regions are indicated as UL and US. Repeat regions are indicated as a, b and c sequences, where primes designate inverted orientations. Sequences ab and b′a′ correspond to the terminal/internal repeat long (TRL/IRL); sequences a′c′ and ca correspond to the internal/terminal repeat short (IRS/TRS). Top: typical genome arrangement of wild-type strains; bottom: genome arrangement of strain AD169, a laboratory-adapted strain. Genome rearrangements that have occurred during extensive passaging are indicated in red between the wild-type and laboratory-adapted configurations.[14]

Herpesviruses have some of the largest genomes among human viruses, often encoding hundreds of proteins. For instance, the double‑stranded DNA (dsDNA) genome of wild-type HCMV strains has a size of around 235 kb and encodes at least 208 proteins. It is thus longer than all other human herpesviruses and one of the longest genomes of all human viruses in general. It has the characteristic herpesvirus class E genome architecture, consisting of two unique regions (unique long UL and unique short US), both flanked by a pair of inverted repeats (terminal/internal repeat long TRL/IRL and internal/terminal repeat short IRS/TRS). Both sets of repeats share a region of a few hundred bps, the so-called "a sequence"; the other regions of the repeats are sometimes referred to as "b sequence" and "c sequence".[14]

Life cycle

Viral replication is nuclear and

DNA templated transcription, with some alternative splicing mechanism is the method of transcription. Translation takes place by leaky scanning. The virus exits the host cell by nuclear egress, and budding. Humans and monkeys serve as the natural hosts. Transmission routes are dependent on coming into contact with bodily fluids (such as saliva, urine, and genital secretions) from an infected individual.[4][15]

Genus Host details Tissue tropism Entry details Release details Replication site Assembly site Transmission
Cytomegalovirus humans; monkeys Epithelial mucosa Glycoproteins Budding Nucleus Nucleus Urine; saliva; congenital

All

herpesviruses share a characteristic ability to remain latent within the body over long periods. Although they may be found throughout the body, CMV infections are frequently associated with the salivary glands in humans and other mammals.[9]

Genetic engineering

The CMV promoter is commonly included in vectors used in genetic engineering work conducted in mammalian cells, as it is a strong promoter and drives constitutive expression of genes under its control.[16]

History

Cytomegalovirus was first observed by German pathologist Hugo Ribbert in 1881 when he noticed enlarged cells with enlarged nuclei present in the cells of an infant.[17] Years later, between 1956 and 1957, Thomas Huckle Weller together with Smith and Rowe independently isolated the virus, known thereafter as "cytomegalovirus".[18] In 1990, the first draft of human cytomegalovirus genome was published,[19] the biggest contiguous genome sequenced at that time.[20]

See also

References

  1. PMID 11002765
    .
  2. ^ Francki RI, Fauquet CM, Knudson DL, Brown (1991). "Classification and nomenclature of viruses. Fifth Report of the International Committee on Taxonomy of Viruses" (PDF). Arch. Virol.: 107.
  3. PMID 28838031
    .
  4. ^ a b c "Viral Zone". ExPASy. Retrieved 15 June 2015.
  5. ^ a b "Virus Taxonomy: 2022 Release". International Committee on Taxonomy of Viruses (ICTV). March 2023. Retrieved 16 September 2022.
  6. ^ "Often overlooked, a common infection during pregnancy kickstarts a conversation about newborn screening". STAT. 5 April 2023. Retrieved 5 April 2023.
  7. ^
    ISBN 978-0-8385-8529-0
    .
  8. PMID 30258007
    .
  9. ^
    ISBN 978-0-521-82714-0
    .
  10. ^ "Panine betaherpesvirus 2 (Chimpanzee cytomegalovirus)". www.uniprot.org. Retrieved 13 March 2019.
  11. ^ "Simian cytomegalovirus (strain Colburn)". www.uniprot.org. Retrieved 13 March 2019.
  12. ^ "Macacine betaherpesvirus 3 (Rhesus cytomegalovirus)". www.uniprot.org. Retrieved 13 March 2019.
  13. ^ "Murid herpesvirus 1, complete genome". 13 August 2018. Retrieved 13 March 2019 – via NCBI Nucleotide.
  14. ^
    PMID 24603756
    .
  15. PMID 21674676
    .
  16. ^ Morgan K (3 April 2014). "Plasmids 101: The Promoter Region – Let's Go!". Addgene Blog.
  17. ISBN 9781904455028
    .
  18. S2CID 29263626
    .
  19. PMID 2161319
    .
  20. PMID 30604286
    .

External links

Wikimedia Commons has media related to Cytomegalovirus.
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